Human ROCK2 Knockdown Cell Lysate

• Cat.No.: ROCK2-290HKCL
• Product Overview: WB-validated ROCK2 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 161 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg ROCK2 KD HeLa cell lysate
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: ROCK2 Rho-associated, coiled-coil containing protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ROCK2
• Synonyms: ROCK2; Rho-associated, coiled-coil containing protein kinase 2; rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II; ROCK-II; KIAA0619
• Gene ID: 9475
• mRNA Refseq: NM_004850
• Protein Refseq: NP_004841
• MIM: 604002
• UniProt ID: O75116