Recombinant Human ROCK2

• Cat.No.: ROCK2-30955TH
• Product Overview: Recombinant fusion protein, corresponding to amino acids 5-554 of Human ROCK2 with an N-terminal tag, expressed by baculovirus in Sf9 insect cells.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
• Biological activity: Specific activity 350 nmol/min/mg.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Contains 1 AGC-kinase C-terminal domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.Contains 1 REM (Hr1) repeat.
• Tag: Non
• Protein length: 5-554

Gene Information

• Gene Name: ROCK2 Rho-associated, coiled-coil containing protein kinase 2 [ Homo sapiens ]
• Official Symbol: ROCK2
• Synonyms: ROCK2; Rho-associated, coiled-coil containing protein kinase 2; rho-associated protein kinase 2
• Gene ID: 9475
• mRNA Refseq: NM_004850
• Protein Refseq: NP_004841
• MIM: 604002
• Uniprot ID: O75116
• Chromosome Location: 2p24
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem
• Function: ATP binding; metal ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity

Reviews

08/19/2021

Swift and hassle-free delivery ensured our experiments stayed on track.

01/13/2020

Efficient customer service provided valuable insights for our decision-making.

07/06/2019

Reliable and precise—this recombined protein never disappoints.

Q&As

Discuss the role of ROCK2 in smooth muscle contraction and vascular tone.

ROCK2 is a key regulator of smooth muscle contraction, influencing vascular tone by phosphorylating myosin light chain and promoting actin-myosin interaction.

Describe the molecular crosstalk between ROCK2 and other signaling pathways.

ROCK2 interacts with various signaling pathways, including Wnt, PI3K/Akt, and MAPK pathways, forming a network that regulates cell survival, proliferation, and differentiation.

How does ROCK2 contribute to the regulation of cell cycle progression?

ROCK2 can influence the cell cycle by regulating cyclin-dependent kinase activity and promoting the phosphorylation of proteins involved in cell cycle checkpoints.

What are the implications of ROCK2 in cell migration and invasion?

ROCK2 is involved in the regulation of cell migration and invasion by controlling actin dynamics and focal adhesion turnover, influencing processes like metastasis in cancer.

Discuss the role of ROCK2 in the regulation of the actin cytoskeleton.

ROCK2 phosphorylates various downstream targets, such as myosin light chain, leading to actin-myosin contraction and the reorganization of the actin cytoskeleton.