Active Recombinant Human ROCK2 Protein, GST tagged

• Cat.No.: ROCK2-421H
• Product Overview: Recombinant human ROCK2 (5-554) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 5-554 aa
• Description: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 370 nmol/min/mg
• Molecular Mass: ~88kDa
• Purity: > 90%
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Shipping: Dry ice
• Reference: 1. Zhao, Z. et al: Rho-associated kinases play a role in endocardial cell differentiation and migration. Dev Biol. 2004 Nov 1;275(1):183-91. 2. Noma, K. et al: Physiological role of ROCKs in the cardiovascular system. Am J Physiol Cell Physiol. 2006 Mar;290(3):C661-8.

Gene Information

• Gene Name: ROCK2 Rho-associated, coiled-coil containing protein kinase 2 [ Homo sapiens ]
• Official Symbol: ROCK2
• Synonyms: ROCK2; Rho-associated, coiled-coil containing protein kinase 2; rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II; ROCK-II; KIAA0619
• Gene ID: 9475
• mRNA Refseq: NM_004850
• Protein Refseq: NP_004841
• MIM: 604002
• UniProt ID: O75116