Recombinant Human ROCK2, GST-tagged, Active

• Cat.No.: ROCK2-421H
• Product Overview: Recombinant human ROCK2 (5-554) was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=88kDa.

Specification

• Cat. No.: ROCK2-421H
• Description: ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. ROCK2 is an immediate downstream target of the small GTPase RhoA. ROCK2 may play a pivotal role in cardiovascular diseases such as vasospastic angina, ischemic stroke, and heart failure. Inhibition of ROCKs by statins or other selective inhibitors leads to the upregulation and activation of endothelial nitric oxide synthase (eNOS) and reduction of vascular inflammation and atherosclerosis.
• Source: Sf9 insect cells using baculovirus.
• Sequence: 5-554.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Protein length: 5-554

Gene Information

• Gene Name: ROCK2 Rho-associated, coiled-coil containing protein kinase 2 [ Homo sapiens ]
• Synonyms: ROCK2; Rho-associated, coiled-coil containing protein kinase 2; KIAA0619; EC 2.7.11.1
• Gene ID: 9475
• mRNA Refseq: NM_004850
• Protein Refseq: NP_004841
• MIM: 604002
• UniProt ID: O75116
• Chromosome Location: 2p24
• Pathway: Axon guidance; Chemokine signaling pathway; Focal adhesion; Leukocyte transendothelial migration; Pathogenic Escherichia coli infection; Regulation of actin cytoskeleton; TGF-beta signaling pathway; Vascular smooth muscle contraction; Wnt signaling pathway; Signaling by GPCR; Axon guidance
• Function: ATP binding; GTP-Rho binding; identical protein binding; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity; zinc ion binding; transferase activity

Reviews

08/19/2021

Swift and hassle-free delivery ensured our experiments stayed on track.

01/13/2020

Efficient customer service provided valuable insights for our decision-making.

07/06/2019

Reliable and precise—this recombined protein never disappoints.

Q&As

Discuss the role of ROCK2 in smooth muscle contraction and vascular tone.

ROCK2 is a key regulator of smooth muscle contraction, influencing vascular tone by phosphorylating myosin light chain and promoting actin-myosin interaction.

Describe the molecular crosstalk between ROCK2 and other signaling pathways.

ROCK2 interacts with various signaling pathways, including Wnt, PI3K/Akt, and MAPK pathways, forming a network that regulates cell survival, proliferation, and differentiation.

How does ROCK2 contribute to the regulation of cell cycle progression?

ROCK2 can influence the cell cycle by regulating cyclin-dependent kinase activity and promoting the phosphorylation of proteins involved in cell cycle checkpoints.

What are the implications of ROCK2 in cell migration and invasion?

ROCK2 is involved in the regulation of cell migration and invasion by controlling actin dynamics and focal adhesion turnover, influencing processes like metastasis in cancer.

Discuss the role of ROCK2 in the regulation of the actin cytoskeleton.

ROCK2 phosphorylates various downstream targets, such as myosin light chain, leading to actin-myosin contraction and the reorganization of the actin cytoskeleton.