Active Recombinant Human CASP8 Protein
Cat.No. : | CASP8-26606TH |
Product Overview : | Recombinant human CASP8 protein without tag was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. The recombinant active human caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested for its ability to enzymatically cleave these two substrates Ac-IETD-pNA or Ac-IETD-AFC. |
Source : | E. coli |
Species : | Human |
Form : | Lyophilized powder |
Bio-activity : | Protein activity determined to be > 5000 units/mg. One unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37 centigrade in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT. |
Purity : | Purity was assessed by SDS-PAGE (≥95%) and by HPLC. |
Applications : | Apuri, Blocking, ELISA, Functional Assay |
Notes : | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Storage : | Store at -80 centigrade. |
Storage Buffer : | Reconstitute to 1 unit per µl in PBS containing 15% glycerol. |
Gene Name : | CASP8 caspase 8 [ Homo sapiens (human) ] |
Official Symbol : | CASP8 |
Synonyms : | CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; CASP8; caspase 8; caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; ICE-like apoptotic protease 5; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MORT1-associated ced-3 homolog; apoptotic cysteine protease; apoptotic protease Mch-5; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; EC 3.4.22.61 |
Gene ID : | 841 |
mRNA Refseq : | NM_001228 |
Protein Refseq : | NP_001219 |
MIM : | 601763 |
UniProt ID : | Q14790 |
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◆ Lysates | ||
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CASP8-7831HCL | Recombinant Human CASP8 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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