Recombinant Human DDIT3, His-tagged

• Cat.No.: DDIT3-26512TH
• Product Overview: Recombinant Full length Human GADD153 fused with an N terminal His-tag; 189aa, 21 kDa.

Specification

• Description: This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
• Conjugation: HIS
• Source: E. coli
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA
• Sequence Similarities: Belongs to the bZIP family.Contains 1 bZIP domain.

Gene Information

• Gene Name: DDIT3 DNA-damage-inducible transcript 3 [ Homo sapiens ]
• Official Symbol: DDIT3
• Synonyms: DDIT3; DNA-damage-inducible transcript 3; DNA damage-inducible transcript 3 protein; C/EBP zeta; CHOP; CHOP10; GADD153
• Gene ID: 1649
• mRNA Refseq: NM_004083
• Protein Refseq: NP_004074
• MIM: 126337
• Uniprot ID: P35638
• Chromosome Location: 12q13.1-q13.2
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Activation of Chaperones by ATF6-alpha, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem
• Function: DNA binding; protein dimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription corepressor activity