Recombinant Human FASN, His-tagged

• Cat.No.: FASN-28809TH
• Product Overview: Recombinant fragment, corresponding to amino acids 2181-2502 of Human Fatty Acid Synthase with an N terminal His tag; MWt 37 kDa

Specification

• Description: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Ubiquitous. Prominent expression in brain, lung, and liver.
• Form: Lyophilised:Reconstitute with 64 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: QLTLRKLQELSSKADEASELACPTP KEDGLAQQQTQLNLR SLLVNPEGPTLMRLNSVQSSERPLF LVHPIEGSTTVFH SLASRLSIPTYGLQCTRAAPLDSIH SLAAYYIDCIRQV QPEGPYRVAGYSYGACVAFEMCSQL QAQQSPAPTHNSL FLFDGSPTYVLAYTQSYRAKLTPGC EAEAETEAICFFVQQFTDMEHNRVL EALLPLKGLEERVAAAVDLIIKSHQ GLD RQELSFAARSFYYKLRAAEQYTPKA KYHGNVMLLRAKT GGAYGEDLGADYNLSQVCDGKVSVH VIEGDHRTLLEGS GLESIISIIHSSLA
• Sequence Similarities: Contains 1 acyl carrier domain.

Gene Information

• Gene Name: FASN fatty acid synthase [ Homo sapiens ]
• Official Symbol: FASN
• Synonyms: FASN; fatty acid synthase; FAS; SDR27X1; short chain dehydrogenase/reductase family 27X; member 1
• Gene ID: 2194
• mRNA Refseq: NM_004104
• Protein Refseq: NP_004095
• MIM: 600212
• Uniprot ID: P49327
• Chromosome Location: 17q25
• Pathway: ChREBP activates metabolic gene expression, organism-specific biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; Fatty Acyl-CoA Biosynthesis, organism-specific biosystem; Fatty acid biosynthesis, organism-specific biosystem; Fatty acid biosynthesis, conserved biosystem
• Function: 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; [acyl-carrier-protein] S-acetyltransferase activity; [acyl-carrier-protein] S-