Native Human GPT

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Native Human GPT

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Cat.No. : GPT-26882TH
Product Overview : Full length Alanine Transaminase from human heart.
Description : This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
Tissue specificity : Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
Biological activity : Specific activity:29 U/mg protein.
Form : Lyophilised
Storage buffer : Preservative: NoneConstituents: 100mM Sodium chloride, 10mM Tris HCl, 1mM EDTA, 1mM DTE, 10mM alpha-Ketoglutarate, 20μg/ml Pyridoxal-5-phosphate, pH 7.0
Storage : Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sequence Similarities : Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
Gene Name : GPT glutamic-pyruvate transaminase (alanine aminotransferase) [ Homo sapiens ]
Official Symbol : GPT
Synonyms : GPT; glutamic-pyruvate transaminase (alanine aminotransferase); alanine aminotransferase 1; ALT1; GPT1;
Gene ID : 2875
mRNA Refseq : NM_005309
Protein Refseq : NP_005300
MIM : 138200
Uniprot ID : P24298
Chromosome Location : 8q24.2-qter
Pathway : Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Metabolic pathways, organism-specific biosystem;
Function : 1-aminocyclopropane-1-carboxylate synthase activity; L-alanine:2-oxoglutarate aminotransferase activity; L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding; transaminase activity;

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