Recombinant Human MAP4K4

• Cat.No.: MAP4K4-27603TH
• Product Overview: Recombinant fragment corresponding to amino acids 1-328 of Human MAP4K4 with N-terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system, MWt 64kDa.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Protein Length: 328 amino acids
• Description: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Molecular Weight: 64.000kDa inclusive of tags
• Biological activity: The specific activity of MAP4K4-27603TH was determined to be 54 nmol/min/mg.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: MAP4K4 mitogen-activated protein kinase kinase kinase kinase 4 [ Homo sapiens ]
• Official Symbol: MAP4K4
• Synonyms: MAP4K4; mitogen-activated protein kinase kinase kinase kinase 4; FLH21957; hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; NIK
• Gene ID: 9448
• mRNA Refseq: NM_001242559
• Protein Refseq: NP_001229488
• MIM: 604666
• Uniprot ID: O95819
• Chromosome Location: 2q11.2-q12
• Pathway: Ceramide signaling pathway, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; EPHB forward signaling, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; small GTPase regulator activity