Recombinant Human MCM3, His-tagged

• Cat.No.: MCM3-28038TH
• Product Overview: Recombinant fragment, corresponding to amino acids 182-808 of Human MCM3 with an N-terminal His tag; Predicted MWt 71kDa.

Specification

• Description: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 84 μl aqua dest
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: NPLETEYGLSVYKDHQTITIQEMPE KAPAGQLPRSVDVIL DDDLVDKAKPGDRVQVVGTYRCLPG KKGGYTSGTFRTV LIACNVKQMSKDAQPSFSAEDIAKI KKFSKTRSKDIFD QLAKSLAPSIHGHDYVKKAILCLLL GGVERDLENGSHI RGDINILLIGDPSVAKSQLLRYVLC TAPRAIPTTGRGSSG VGLTAAVTTDQETGERRLEAGAMVL ADRGVVCIDEFDK MSDMDRTAIHEVMEQGRVTIAKAGI HARLNARCSVLAA ANPVYGRYDQYKTPMENIGLQDSLL SRFDLLFIMLDQM DPEQDREISDHVLRMHRYRAPGEQD GDAMPLGSAVDILAT DDPNFSQEDQQDTQIYEKHDNLLHG TKKKKEKMVSAAF MKKYIHVAKIIKPVLTQESATYIAE EYSRLRSQDSMSS DTARTSPVTARTLETLIRLATAHAK ARMSKTVDLQDAE EAVELVQYAYFKKVLEKEKKRKKRS EDESETEDEEEKSQEDQEQKRKRRK TRQPDAKDGDSYDPYDFSDTEEEMP QVH TPKTADSQETKESQKVELSESRLKA FKVALLDVFREAH AQSIGMNRLTESINRDSEEPFSSVE IQAALSKMQDDNQ VMVSEGIIFLI
• Sequence Similarities: Belongs to the MCM family.Contains 1 MCM domain.

Gene Information

• Gene Name: MCM3 minichromosome maintenance complex component 3 [ Homo sapiens ]
• Official Symbol: MCM3
• Synonyms: MCM3; minichromosome maintenance complex component 3; MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) , minichromosome maintenance deficient (S. cerevisiae) 3; DNA replication licensing factor MCM3
• Gene ID: 4172
• mRNA Refseq: NM_002388
• Protein Refseq: NP_002379
• MIM: 602693
• Uniprot ID: P25205
• Chromosome Location: 6p12
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Activation of the pre-replicative complex, organism-specific biosystem; Assembly of the pre-replicative complex, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem
• Function: ATP binding; DNA binding; helicase activity; hydrolase activity; nucleotide binding