Recombinant Human NCALD, His-tagged

• Cat.No.: NCALD-27853TH
• Product Overview: Recombinant full length Human NCALD , with an N terminal His tag ; predicted mwt: 24.4 kDa.

Specification

• Description: This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.
• Protein length: 193 amino acids
• Conjugation: HIS
• Molecular Weight: 24.400kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Retina, cerebrum, cerebellum, brain stem, spinal cord, testis, ovary and small intestine.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 40% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, pH 8
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFRTFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNRDGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF
• Sequence Similarities: Belongs to the recoverin family.Contains 4 EF-hand domains.

Gene Information

• Gene Name: NCALD neurocalcin delta [ Homo sapiens ]
• Official Symbol: NCALD
• Synonyms: NCALD; neurocalcin delta; neurocalcin-delta
• Gene ID: 83988
• mRNA Refseq: NM_001040624
• Protein Refseq: NP_001035714
• MIM: 606722
• Uniprot ID: P61601
• Chromosome Location: 8q22-q23
• Pathway: Activation of Ca-permeable Kainate Receptor, organism-specific biosystem; Activation of Kainate Receptors upon glutamate binding, organism-specific biosystem; Ionotropic activity of Kainate Receptors, organism-specific biosystem; Neuronal System, organism-specific biosystem; Neurotransmitter Receptor Binding And Downstream Transmission In ThePostsynaptic Cell, organism-specific biosystem
• Function: actin binding; calcium ion binding; clathrin binding; tubulin binding