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Recombinant Human TNFSF8

Cat. No.: TNFSF8-1595H
Product Overview: The sequence encoding the signal peptide from human CD33 and a linker peptide was fused to the extracellular domain of human CD30L, inserted into a suitable mammalian expression vector and expressed inNSO cells.
Description: The CD30 ligand is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin"s disease derived cell lines. The receptor for CD30L, CD30/ TNFRSF8/CD153, is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.
Molecular Weight: The predicted molecular weight of Recombinant Human CD30 Ligand is Mr 22 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 30-40 kDa.
Source: NSO Cells.
State Of Matter: Lyophilized.
Purity: >95% by SDS Page and analyzed by silver stain.
Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Endotoxin: <1.0 EU/µg as determined by the LAL method.
Biological Activity: The biological activity of Human CD30L was determined by its ability to stimulate IL-6 secretion by HDLM cells, a Hodgkin's lymphoma cell line (Gruss, H-J. et al., 1996, Blood 87:2443). The expected ED50 for this effect is typically 0.1 - 0.4 μg/mL in the presence of 10 μg/mL of a cross-linking antibody Recombinant Rat Resistin + His-tag.
Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Gene Name: TNFSF8 tumor necrosis factor (ligand) superfamily, member 8 [ Homo sapiens ]
Synonyms: TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; CD153; CD30-L; CD30L; CD30LG; MGC138144; CD153 antigen; CD30 antigen ligand; CD30 ligand; OTTHUMP00000022762
Gene ID: 944
mRNA Refseq: NM_001244
Protein Refseq: NP_001235
MIM: 603875
UniProt ID: P32971
Chromosome Location: 9q33
Pathway: kine-cytokine receptor interaction
Function: cytokine activity; tumor necrosis factor receptor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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