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Recombinant Human Glutaredoxin 3, His-tagged

Cat. No.: GLRX3-2405H
Product Overview: Recombinant human Glutaredoxin-3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 39.6kDa (355aa).
Description: Glutaredoxin(GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (Grx3/PICOT) is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals. And it has been shown to interact with PRKCQ.
Source: E.coli.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT.
Purity: > 95% by SDS – PAGE.
Concentration: 0.5mg/ml (determined by Bradford assay).
Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN.
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: GLRX3 glutaredoxin 3 [ Homo sapiens ]
Synonyms: glutaredoxin 3; GRX3; GRX4; GLRX4; PICOT; TXNL2; TXNL3; FLJ11864; bA500G10.4; GLRX3; glutaredoxin-3; glutaredoxin 4; thioredoxin-like 2; PKCq-interacting protein; thioredoxin-like protein 2; PKC-theta-interacting protein; PKC-interacting cousin of thioredoxin; OTTHUMP00000020747; Thioredoxin-like protein 2
Gene ID: 10539
mRNA Refseq: NM_006541
Protein Refseq: NP_006532
MIM: 612754
UniProt ID: O76003
Chromosome Location: 10q26
Function: electron carrier activity; protein binding; protein disulfide oxidoreductase activity; protein kinase C binding

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