||Recombinant FTH1 protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 21kDa (183aa).
||Ferritin, heavy polypeptide 1 (FTH1) is a ubiquitous protein which plays a major role in iron sequestration, detoxification and storage. FTH1 is found in all living species. FTH1 encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains.
|Amino Acid Sequence:
||MTTASTSQVR QNYHQDSEAA INRQINLELY ASYVYLSMSY YFDRDDVALK NFAKYFLHQS HEEREHAEKL MKLQNQRGGR IFLQDIKKPD CDDWESGLNA MECALHLEKN VNQSLLELHK LATDKNDPHL CDFIETHYLN EQVKAIKELG DHVTNLRKMG APESGLAEYL FDKHTLGDSD NES.
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol.
||> 95% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Porphyrin and chlorophyll metabolism; Membrane Trafficking