Recombinant Human FTH1 protein

• Cat.No.: FTH1-528H
• Product Overview: Recombinant Human FTH1 protein(P02794) (Met 1-Ser183) was expressed and purified in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-183 aa
• Form: Lyophilized from sterile PBS, pH 7.5.
• Molecular Mass: The recombinant human FTH1 consists of 183 amino acids and has a calculated molecular mass of 21.2KDa as estimated in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections (2022)

Gene Information

• Gene Name: FTH1 ferritin, heavy polypeptide 1 [ Homo sapiens ]
• Official Symbol: FTH1
• Synonyms: FTH1; ferritin, heavy polypeptide 1; FTHL6; ferritin heavy chain; apoferritin; FHC; FTH; PIG15; placenta immunoregulatory factor; PLIF; proliferation inducing protein 15; ferritin H subunit; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein; MGC104426
• Gene ID: 2495
• mRNA Refseq: NM_002032
• Protein Refseq: NP_002023
• UniProt ID: P02794

Citation

Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections

Journal: Nature Communications    PubMed ID: 35091550    Data: 2022/1/28

Authors: Brady T. Hickerson, Tracy R. Daniels-Wells, Brian B. Gowen

Article Snippet:Erythroblasts (5 × 10 5 ) were incubated with 5 μg human diferric Tf conjugated to Alexa Fluor? 546 (Thermo Fisher Scientific, Cat. No. T23364) or 3 μg of recombinant H-Ft conjugated to R-phycoerythrin (H-Ft-PE; Creative BioMart, Shirley, NY, USA, Cat. No. FTH1-528H-PE) for 60 min on ice.. Competing antibodies were added to some samples and included 10 μg of either anti-dansyl IgG1/κ isotype control antibody , ch128.1/IgG1, or the murine anti-hTfR1 IgG2a/κ antibody clone M-A712 (BD Biosciences, Cat. No. 555534) that is known to block H-Ft binding .Competing antibodies were added to some samples and included 10 μg of either anti-dansyl IgG1/κ isotype control antibody , ch128.1/IgG1, or the murine anti-hTfR1 IgG2a/κ antibody clone M-A712 (BD Biosciences, Cat. No. 555534) that is known to block H-Ft binding .