||Recombinant Bovine IL-4 was expressed inYeast. MW= 12.6 kDa (112 aa).
||Interleukin-4, abbreviated IL-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. Its receptor is the Interleukin-4 receptor.
||Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
||>95% as visualized by SDS-PAGE analysis.
|Amino Acid Sequence:
||GHKCDITLAE IIKTLNILTT RKNSCMELPV ADVFAAPKNT TEKETFCRVG IELRRIYRSH TCLNKFLGGL DRNLNSLASK TCSVNEAKTS TSTLKDLLER LKTIMKEKYS KC.
||The biological activity of recombinant bovine IL-4 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 1.5 - 2 ng/mL.
|Stability And Storage:
||Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.