Active Recombinant Mouse Efna1 protein(Met1-Ser182), hFc-tagged

• Cat.No.: Efna1-3264M
• Product Overview: Recombinant Mouse EFNA1 (NP_034237.3)(Met 1-Ser 182) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Ser182
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA2 at 2 μg/ml (100 μl/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/ml.
• Molecular Mass: The secreted recombinant mouse EFNA1/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 405 amino acids and has a predicted molecular mass of 46.4 kDa. As a result of glycosylation, the apparent molecular mass of rm EFNA1/Fc monomer is approximately 53 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Efna1 ephrin A1 [ Mus musculus ]
• Official Symbol: Efna1
• Synonyms: EFNA1; ephrin A1; ephrin-A1; immediate early response protein B61; EPH-related receptor tyrosine kinase ligand 1; B61; Efl1; Epl1; Eplg1; Lerk1; AI325262
• Gene ID: 13636
• mRNA Refseq: NM_001162425
• Protein Refseq: NP_001155897