Active Recombinant Human EFNA1 protein, Fc-His-tagged

• Cat.No.: EFNA1-2326H
• Product Overview: Active Recombinant Human EFNA1 protein(NP_004419.2)(Met1-Ser182) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-182 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EphA2 at 2 μg/ml (100 μl/well) can bind human EphrinA1 with a linear range of 0.8-20 ng/ml.
• Molecular Mass: The recombinant human EphrinA1/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 412 amino acids a predicted molecular mass of 47.5 kDa. As a result of glycosylation, rh EphrinA1/Fc monomer migrates as an apximately 50-55 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_004419.2
• Unit ID: P20827
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P20827

Gene Information

• Gene Name: EFNA1 ephrin-A1 [ Homo sapiens ]
• Official Symbol: EFNA1
• Synonyms: EFNA1; ephrin-A1; EPLG1, TNFAIP4; ECKLG; LERK1; TNF alpha-induced protein 4; ligand of eph-related kinase 1; immediate early response protein B61; eph-related receptor tyrosine kinase ligand 1; tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4; B61; EFL1; EPLG1; LERK-1; TNFAIP4
• Gene ID: 1942
• mRNA Refseq: NM_004428
• protein Refseq: NP_004419
• MIM: 191164