Active Recombinant Human KNG1 protein, His-tagged

• Cat.No.: KNG1-3179H
• Product Overview: Recombinant Human KNG1 protein(NP_001095886.1)(Gln19-Ser644), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 25mM Tris, 100mM NaCl, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is < 7 nM.
• Molecular Mass: The recombinant human KNG1 consists of 637 amino acids and has a predicted molecular mass of 71.3 kDa. As a result of glycosylation, the apparent molecular mass of rhKNG1 is approximately 100-110 kDa in SDS-PAGE under reducing conditions.
• Protein Length: Gln19-Ser644
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KNG1 kininogen 1 [ Homo sapiens ]
• Official Symbol: KNG1
• Synonyms: KNG1; kininogen 1; BDK, kininogen , KNG; kininogen-1; alpha 2 thiol proteinase inhibitor; bradykinin; HMWK; fitzgerald factor; high molecular weight kininogen; alpha-2-thiol proteinase inhibitor; williams-Fitzgerald-Flaujeac factor; BDK; KNG
• Gene ID: 3827
• mRNA Refseq: NM_000893
• Protein Refseq: NP_000884
• MIM: 612358
• UniProt ID: P01042