Active Recombinant Human SIRPA protein, His-tagged
Cat.No. : | SIRPA-3218H |
Product Overview : | Recombinant Human SIRPA protein(NP_542970.1)(Met1-Arg370, Gly 75 Ala), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | Immobilized Recombinant Human CD47 Protein (ECD,Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human SIRP alpha / CD172a Protein (ECD, His Tag), the EC50 is 280-850 ng/mL. |
Molecular Mass : | The recombinant human SIRPA consists of 351 amino acids and predictes a molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human SIRPA is approximately 45-55 kDa due to glycosylation. |
Proteinlength : | Met1-Arg370,Gly75Ala |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 98 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | SIRPA signal-regulatory protein alpha [ Homo sapiens ] |
Official Symbol : | SIRPA |
Synonyms : | SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1; |
Gene ID : | 140885 |
mRNA Refseq : | NM_001040022 |
Protein Refseq : | NP_001035111 |
MIM : | 602461 |
UniProt ID : | P78324 |
Products Types
◆ Recombinant Protein | ||
SIRPA-1251M | Acitve Recombinant Mouse SIRPA protein(Met1-Asn373), His-tagged | +Inquiry |
SIRPA-5066R | Recombinant Rat SIRPA Protein, His (Fc)-Avi-tagged | +Inquiry |
SIRPA-13H | Active Recombinant Human SIRPA Protein, His-tagged | +Inquiry |
SIRPA-723H | Recombinant Human SIRPA Protein, Fc-tagged | +Inquiry |
SIRPA-515H | Recombinant Human SIRPA Protein (Met1-Arg370), MIgG2a Fc-tagged | +Inquiry |
◆ Lysates | ||
SIRPA-1650MCL | Recombinant Mouse SIRPA cell lysate | +Inquiry |
SIRPA-2298HCL | Recombinant Human SIRPA cell lysate | +Inquiry |
SIRPA-1005RCL | Recombinant Rat SIRPA cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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