Recombinant Human Replication Protein A2, 32kDa, His-tagged

• Cat.No.: RPA2-2852H
• Product Overview: Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =31.7 kDa (293aa) confirmed by MALDI-TOF.

Specification

• Cat. No.: RPA2-2852H
• Description: Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. The C-terminus of RPA2 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, this protein binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.
• Source: E.coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMWNSGFE SYGSSSYGGA GGYTQSPGGF GSPAPSQAEK KSRARAQHIV PCTISQLLSA TLVDEVFRIG NVEISQVTIV GIIRHAEKAP TNIVYKIDDM TAAPMDVRQW VDTDDTSSEN TVVPPETYVK VAGHLRSFQN KKSLVAFKIM PLEDMNEFTT HILEVINAHM VLSKANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Purity: > 85 % by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RPA2 replication protein A2, 32kDa [ Homo sapiens ]
• Synonyms: RPA2; replication protein A2, 32kDa; REPA2; RPA32; replication protein A 32 kDa subunit; RP-A p32; RP-A p34; RF-A protein 2; OTTHUMP00000004010; replication factor A protein 2; replication protein A 34 kDa subunit; RPA34; replication protein A2 (32kD)
• Gene ID: 6118
• mRNA Refseq: NM_002946
• Protein Refseq: NP_002937
• MIM: 179836
• UniProt ID: P15927
• Chromosome Location: 1p35
• Pathway: DNA replication; Homologous recombination; Mismatch repair; Nucleotide excision repair
• Function: nucleic acid binding; protein binding; single-stranded DNA binding