||Recombinant Human Interferon, Alpha 2 was expressed inPseudomonas putida. MW = 19.2 kDa.
||Interferon-α (INF-α), also known as leukocyte interferon, comprises a grope of related but distinct proteins that share over 95 % amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, 100 kDa ligand-binding subunit (INF-αR1) and a 125 kDa subunit (INF-αR2) that is involved both in ligand binding and signal transduction. INF-α has both anti-viral and immunomodulatory activities on target cells.
||>98% determined by HPLC and PAGE.
||lyophilised with carrier.
||>2.5 x 10E+8 unit/mg was determined in the cytopathic effect inhibition assay using human diploid fibroblast cells with vesicular stomatitis virus (VSV). A cytopathic effect of 50% was produced with 1 unit/ml interferon. The units are determined according to the international reference standard provided by WHO.
||protein <2% (HPLC), DNA <50pg/mg of protein.