||Recombinant rat ApoJ/Clusterin was constructed with an Nterminal T7-Tag fusion (16AA) and a C-terminal His-Tag fusion (9AA). This His-tagged fusion protein produced inE.colihas a molecular weight of 26.5 kDa containing 215 amino acid residues of the APO-J Rat and 25 additional amino acid residues (His-Tag, T7-Tag).
||Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein. Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%.
||Greater than 95% (determined by SDS-PAGE and HPLC analysis).
||Elisa, Western blotting.
||Lyophilized (freeze-dried), sterile powder. Lyophilized from a concentrated (0.5 mg/ml) protein solution in 0.01 M Tris (pH 7.2).
||Please Note: Always centrifuge product briefly before opening vial. Add 0.2 ml of deionized, sterile H2O and let the lyophilized pellet dissolve completely.This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
||The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.