Recombinant Human Polycystic Kidney Disease 2 (Autosomal Dominant)

• Cat.No.: PKD2-660H

Specification

• Cat. No.: PKD2-660H
• Description: PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. It exhibits the strongest homology to the serine/threonine protein kinases PKD/PKCμ and PKCν. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation.
• Source: E.coli.
• Application: Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
• Biochem/physiol Actions: PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation.
• Physical Form: Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.
• Assay: ≥70% (SDS-PAGE).
• Form: aqueous glycerol solution.
• Mol Wt: protein apparent mol wt ~140 kDa.
• Storage Temp: −70°C.

Gene Information

• Gene Name: PKD2 polycystic kidney disease 2 (autosomal dominant) [ Homo sapiens ]
• Synonyms: PKD2; polycystic kidney disease 2 (autosomal dominant); PC2; PKD4; Pc-2; APKD2; TRPP2; MGC138466; MGC138468; polycystwin; polycystwin; transient receptor potential cation channel, subfamily P, member 2
• Gene ID: 5311
• mRNA Refseq: NM_000297
• Protein Refseq: NP_000288
• MIM: 173910
• UniProt ID: Q13563
• Chromosome Location: 4q21-q23
• Function: ATP binding; calcium ion binding; channel activity; cytoskeletal protein binding; ion channel activity; potassium channel activity; protein C-terminus binding; voltage-gated chloride channel activity; voltage-gated sodium channel activity