||Recombinant Human NFKB1 isproduced inSf21 cellsand fused to a His-tag.
||This gene encodes a 105 kDa protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kDa protein. The 105 kDa protein is a Rel protein-specific transcription inhibitor and the 50 kDa protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.
||Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
||~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
||-80°C. After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.