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GMP Recombinant Human HGF Protein

Cat.No.: HGF-143HG
Product Overview: Recombinant Human HGF, Gln32-Ser728, was produced in NS0 cell under cGMP guidelines.
Source: NS0
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883.
The specific activity of recombinant human HGF is approximately 1.5 x 10^3 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).
Molecular Mass: Predicted Molecular Mass: alpha chain: 53.7; beta chain: 26 kDa
SDS-PAGE: 65-75 kDa, non-reducing conditions
60-65 kDa, and 30-40 kDa, reducing conditions
N-terminal Sequence Analysis: Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32
Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (alpha -chain)
Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (beta -chain)
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
Official Symbol: HGF
Synonyms: HGF; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; F-TCF; DFNB39;
Gene ID: 3082
mRNA Refseq: NM_000601
Protein Refseq: NP_000592
MIM: 142409
UniProt ID: P14210

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