Active GMP Recombinant Human SDC1 protein, Fc-tagged

• Cat.No.: SDC1-187HG
• Product Overview: GMP Recombinant Human SDC1 protein(NP_001006947)(463 aa), fused with Fc Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: DNA sequence encoding Human CD138(UniProtKB/Swiss-Prot: P18827.3) was expressed with Fc tag at the C-terminal.
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: Use immobilized protein to enhance the ability of Saos2 (human osteosarcoma cells) to adhere to human fibronectin. Add 5×104 cells/well to human Syndocan-1 and human fibronectin (0.5ug/ml) coated plates , Cell adhesion was enhanced in a dose-dependent manner. The ED 50 for this effect is 5-10ug/ml.
• Molecular Mass: Recombinant Human SDC1 protein contains 463 aa. It has a predicted MW of 50.8 kDa.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SDC1 syndecan 1 [ Homo sapiens ]
• Official Symbol: SDC1
• Synonyms: SDC1; Syndecan-1; SYND1; SDC
• Gene ID: 6382
• mRNA Refseq: NM_001006946
• Protein Refseq: NP_001006947
• MIM: 186355
• UniProt ID: P18827