|Product Overview:||Recombinant Human TGFB3, Ala301-Ser412, was produced in Sf 9 (baculovirus)-derived in an animal component free process under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.|
|Bio-activity:||Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.01-0.04 ng/mL.
The specific activity of recombinant human TGF-beta 3 is approximately 2.2 x 10^4 IU/μg, which is calibrated against recombinant human TGF-beta 3 WHO International Standard (NIBSC code: 09/234).
|Molecular Mass:||Predicted Molecular Mass: 12.7 (monomer) kDa
SDS-PAGE: 12 kDa, reducing conditions; 24 kDa, non-reducing conditions
|N-terminal Sequence Analysis:||Ala301-Leu-Asp-Thr-Asn-Tyr-(Cys)-Phe-Arg-Asn|
|Endotoxin:||<0.01 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 50-200 μg/mL in sterile 4 mM HCl.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||TGFB3 transforming growth factor, beta 3 [ Homo sapiens ]|
|Synonyms:||TGFB3; transforming growth factor, beta 3; transforming growth factor beta-3; TGF-beta-3; ARVD; TGF-beta3; FLJ16571;|