Human USP14 Knockdown Cell Lysate

• Cat.No.: USP14-465HKCL
• Product Overview: WB-validated USP14 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 56 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg USP14 KD HeLa cell lysate
• Protein Families: Druggable Genome, Protease
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) [ Homo sapiens (human) ]
• Official Symbol: USP14
• Synonyms: USP14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14 (tRNA guanine transglycosylase); ubiquitin carboxyl-terminal hydrolase 14; TGT; ubiquitin thioesterase 14; deubiquitinating enzyme 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; ubiquitin-specific-processing protease 14; tRNA-guanine transglycosylase, 60-kD subunit; ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
• Gene ID: 9097
• mRNA Refseq: NM_001037334
• Protein Refseq: NP_001032411
• MIM: 607274
• UniProt ID: P54578