Mouse Anti-Human Carbonic Anhydrase III, Muscle Specific Monoclonal Antibody
Cat.No. : | CAB11487MH |
Product Overview : | Mouse monoclonal antibody to human carbonic anhydrase III, muscle specific. |
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Antigen Description : | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site.Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. |
Specificity : | Human Carbonic Anhydrase III / CA3. No cross-reactivity in ELISA with Human Carbonic Anhydrase II / CA2, Human Carbonic Anhydrase VA / CA5a, Human Carbonic Anhydrase VIII / CA8, Human Carbonic Anhydrase IX / CA9, Human Carbonic Anhydrase XIII / CA13, Human Carbonic Anhydrase XII / CA12, E.coli cell lysate. |
Immunogen : | Recombinant Human Carbonic Anhydrase III / CA3 Protein |
Host : | Mouse |
Isotype : | Mouse IgG1 |
Species : | Human |
Clone : | 6H9C7 |
Purification : | Protein A affinity chromatography |
Applications : | Western Blot; ELISA |
Dilution : | Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect CA3 in WB. Using a DAB detection system, the detection limit for CA3 is approximately 2 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect CA3. The detection limit for CA3 is approximately 0.16 ng/well. |
Preparation : | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Carbonic Anhydrase III / CA3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Format : | 0.2 μm filtered solution in PBS with 5% trehalose |
Storage : | This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Tag : | Non |
Gene Name : | CA3 carbonic anhydrase III, muscle specific [ Homo sapiens ] |
Official Symbol : | CA3 |
Synonyms : | CA3; carbonic anhydrase III, muscle specific; carbonic anhydrase 3; CAIII; Car3; CA-III; carbonate dehydratase III; FLJ36434; |
Gene ID : | 761 |
mRNA Refseq : | NM_005181 |
Protein Refseq : | NP_005172 |
MIM : | 114750 |
UniProt ID : | P07451 |
Chromosome Location : | 8q21.2 |
Function : | carbonate dehydratase activity; lyase activity; metal ion binding; nickel cation binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
Car3-766M | Recombinant Mouse Car3 Protein, MYC/DDK-tagged | +Inquiry |
CA3-1051H | Recombinant Human CA3 Protein (M1-K260), His/Strep tagged | +Inquiry |
CA3-480H | Recombinant Human CA3 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA3-0239H | Recombinant Human CA3 Protein, GST-Tagged | +Inquiry |
CA3-1159M | Recombinant Mouse CA3 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
CA3-7914HCL | Recombinant Human CA3 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewOutstanding protein sequencing capabilities, unrivaled accuracy.
Precise purification techniques, yield exceptional samples.
Efficient sample handling, optimizes our laboratory workflow.
Q&As (7)
Ask a questionCA3 is primarily found in skeletal muscles.
Post-translational modifications, like phosphorylation or glycosylation, can be identified using mass spectrometry.
Specific inhibitors for CA3 are yet to be fully elucidated and would be determined using enzymatic assays.
CA3 plays a role in CO2 and bicarbonate equilibrium, crucial for metabolic pathways like glycolysis and the citric acid cycle.
While CA3 has a similar mechanism, its activity and affinity might differ from other isoforms, requiring comparative studies.
CA3 dysregulation might be linked to muscle diseases or metabolic disorders, requiring further research.
CA3 catalyzes the reversible hydration of carbon dioxide, aiding in pH regulation and CO2 transport.
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