Recombinant Active Human BMP8A Protein, His-tagged(C-ter)

• Cat.No.: BMP8A-18H
• Product Overview: Recombinant Active Human BMP8A Protein with His tag (C-ter) was expressed in E. coli.

Specification

• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 1.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Powder
• Bio-activity: Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 10-19.4 ng/mL.
• AA Sequence: MAVRPLRRRQPKKSNELPQANRLPG IFDDVHGSHGRQVCRRHELYVSFQD LGWLDWVIAPQGYSAYYCEGECSFP LDSCMNATNHAILQSLVHLMKPNAV PKACCAPTKLSATSVLYYDSSNNVI LRKHRNMVVKACGCH
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 98% (by SDS-PAGE)
• Applications: SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.

Gene Information

• Gene Name: BMP8A bone morphogenetic protein 8a [ Homo sapiens ]
• Official Symbol: BMP8A
• Synonyms: BMP8A; bone morphogenetic protein 8a; bone morphogenetic protein 8A; BMP-8A; FLJ14351; FLJ45264
• Gene ID: 353500
• mRNA Refseq: NM_181809
• Protein Refseq: NP_861525
• UniProt ID: Q7Z5Y6