Recombinant Active Human IL36A Protein, His-tagged(C-ter)

• Cat.No.: IL36A-195H
• Product Overview: Recombinant Active Human IL36A Protein with His tag (C-ter) was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Powder
• Bio-activity: Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 0.7 ng/mL.
• AA Sequence: MKIDTPQQGSIQDINHRVWVLQDQT LIAVPRKDRMSPVTIALISCRHVET LEKDRGNPIYLGLNGLNLCLMCAKV GDQPTLQLKEKDIMDLYNQPEPVKS FLFYHSQSGRNSTFESVAFPGWFIA VSSEGGCPLILTQELGKANTTDFGL TMLF
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 98% (by SDS-PAGE)
• Applications: SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: PBS (pH 7.4)
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.

Gene Information

• Gene Name: IL36A interleukin 36, alpha [ Homo sapiens ]
• Official Symbol: IL36A
• Synonyms: IL36A; interleukin 36, alpha; IL1F6, interleukin 1 family, member 6 (epsilon); interleukin-36 alpha; FIL1; FIL1E; IL 1F6; IL1(EPSILON); MGC129552; MGC129553; FIL1 epsilon; IL-1 epsilon; interleukin-1 epsilon; IL-1F6 (FIL-1-epsilon); interleukin 1, epsilon; interleukin-1 family member 6; interleukin 1 family, member 6 (epsilon); IL1F6; IL-1F6; FIL1(EPSILON)
• Gene ID: 27179
• mRNA Refseq: NM_014440
• Protein Refseq: NP_055255
• MIM: 605509
• UniProt ID: Q9UHA7

Reviews

07/20/2022

When conducting experiments with HSP90AA1, clear and reliable interpretation of results can be obtained.

03/12/2021

The IL36A product has low endotoxin levels and does not interfere with experimental results.

08/12/2020

IL36A has important application value in the field of drug research and development, and has achieved certain research results.

Q&As

Does IL36A carry a fever risk?

In general, the use of IL36A does not cause significant fever reactions, but individual differences exist, if there are abnormalities, please consult a doctor.

How to prepare IL36A?

The IL36A is expressed, purified, and constructed using recombinant DNA technology in a suitable host expression system, usually through genetic engineering.

In what inflammatory diseases does recombinant IL36A have potential applications?

IL36A has potential application value in psoriasis, arthritis and other inflammatory diseases, but the specific efficacy needs further study.

Does IL36A have anti-tumor effects?

IL36A has been shown in some studies to have anti-proliferative and anti-invasive effects on certain tumors, but more studies are needed to confirm its specific mechanism of action and effect.

How is the research progress of IL36A?

The research of IL36A is progressing continuously, and some studies have shown its potential in immune regulation, anti-inflammatory effect and so on.

Can IL36A be used in animal experiments?

Yes, IL36A can be used in animal studies to investigate its mechanism of action and potential applications.