Recombinant Cattle S100A12 protein, His-tagged

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Recombinant Cattle S100A12 protein, His-tagged

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Cat.No. : S100A12-1478C
Product Overview : Recombinant Cattle S100A12 aa. (Thr2~Glu92) fused with N-terminal His tag was produced in E. coli cells.
Source : E. coli
Species : Cattle
Tag : His
Form : Freeze-dried powder
Molecular Mass : 14kDa as determined by SDS-PAGE reducing conditions.
Protein length : Thr2~Glu92
Endotoxin : <1.0EU per 1µg (determined by the LAL method).
Purity : >98%
Characteristic : The isoelectric point is 5.9.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200µg/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : S100A12 S100 calcium binding protein A12 [ Bos taurus (cattle) ]
Official Symbol : S100A12
Synonyms : CAGC; CAAF1; CO-AG; EN-RAGE; RAGE-binding protein; calcium-binding protein in amniotic fluid 1; calgranulin-C; cornea-associated antigen; extracellular newly identified RAGE-binding protein; protein S100-A12
Gene ID : 282467
mRNA Refseq : NM_174651.3
Protein Refseq : NP_777076.1
UniProt ID : P79105

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