Active Recombinant Cynomolgus IFNG Protein

• Cat.No.: IFNG-1165C
• Product Overview: Recombinant cynomolgus IFNG [Identical to rhesus IFNG (NP_001028077.1)] (Gln24-Gln165) was expressed and purified with an initial Met.

Specification

• Source: E. coli
• Species: Cynomolgus
• Predicted N Terminal: Met
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Cynomolgus IFNG at 10 μg/ml (100 μl/well) can bind Cynomolgus IFNGR1-Fc. The EC50 of Cynomolgus IFNGR1-Fc is 20.28-47.32 ng/ml.
• Molecular Mass: The recombinant cynomolgus IFNG consists of 143 amino acids and has a calculated molecular mass of 16.9 kDa. It migrates as an 17 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IFNG interferon, gamma [ Macaca fascicularis ]
• Official Symbol: IFNG
• Synonyms: IFN-gamma; interferon-gamma
• Gene ID: 102128291
• mRNA Refseq: NM_001287657
• Protein Refseq: NP_001274586
• UniProt ID: P63309
• Chromosome Location: chromosome: 11
• Pathway: African trypanosomiasis, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem