Recombinant Cynomolgus IFNG Protein

Cat.No.: IFNG-1165C
Product Overview: Recombinant cynomolgus IFNG [Identical to rhesus IFNG (NP_001028077.1)] (Gln24-Gln165) was expressed and purified with an initial Met.
Source: E. coli
Species: Cynomolgus
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Cynomolgus IFNG at 10 μg/ml (100 μl/well) can bind Cynomolgus IFNGR1-Fc. The EC50 of Cynomolgus IFNGR1-Fc is 20.28-47.32 ng/ml.
Molecular Mass: The recombinant cynomolgus IFNG consists of 143 amino acids and has a calculated molecular mass of 16.9 kDa. It migrates as an 17 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: IFNG interferon, gamma [ Macaca fascicularis ]
Official Symbol: IFNG
Synonyms: IFN-gamma; interferon-gamma
Gene ID: 102128291
mRNA Refseq: NM_001287657
Protein Refseq: NP_001274586
UniProt ID: P63309
Chromosome Location: chromosome: 11
Pathway: African trypanosomiasis, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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