Recombinant Cynomolgus IL33 protein, His-tagged

• Cat.No.: IL33-0291C
• Product Overview: Recombinant Cynomolgus IL33 protein( A0A2K5W3I1-1)(His109-Ile270), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: His109-Ile270
• Tag: N-His
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Human IL-1RL1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-33, His Tag with an affinity constant of 46.85 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.