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Recombinant Cynomolgus LDLR protein, His-tagged

Cat.No.: LDLR-366C
Product Overview: Cynomolgus LDL R, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Gly 788 (Accession # XP_005588053.1).
Description: Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.
Source: HEK293
Species: Cynomolgus
Tag : His
Predicted N Terminal: Ala 22
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.3, 10% trehalose.
Bio-activity: Immobilized Cynomolgus LDL R, His Tag at 2μg/mL (100 μL/well) can bind Biotinylated Human PCSK9, His Tag with a linear range of 0.007-0.256 μg/mL. Immobilized Cynomolgus PCSK9, His Tag at 10μg/mL (100 μL/well) can bind Cynomolgus LDL R, His Tag with a linear range of 0.04-0.256 μg/mL.
Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 86.8 kDa. The protein migrates as 135-155 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length: Ala 22 - Gly 788
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Storage buffer: PBS, pH7.3
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping: Powder,RT
Gene Name: LDLR low density lipoprotein receptor [ Macaca fascicularis (crab-eating macaque) ]
Official Symbol: LDLR
Synonyms: low-density lipoprotein receptor
Gene ID: 102127361
mRNA Refseq: XM_005587996.2
Protein Refseq: XP_005588053.1

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