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Active Recombinant Dog CYP1A1 Protein

Cat.No. : CYP1A1-112D
Product Overview : Recombinant dog CYP1A1 protein and human CYP-reductase without tag were coexpressed in E. coli.
  • Specification
  • Gene Information
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Description : A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).
Source : E. coli
Species : Dog
Bio-activity : Cytochrome c Reductase Activity: 375 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: 7-ethoxycoumarin O-deethylase
Linear with time up to: 6 min(Determined at 5 µM substrate, using a P450 concentration of 0.5 pmol/ml)
Linear with P450 up to: 2.5 pmol/ml(Determined at 5 µM substrate, using an incubation time of 5 min)
Vmax: 8.1 pmol/min/pmol CYP
Km: 7.2 μM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 10.0 nmol/ml
Protein Concentration: 11.0 mg/ml
Specific P450 Content: 906 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CYP1A1 cytochrome P450 family 1 subfamily A member 1 [ Canis lupus familiaris (dog) ]
Official Symbol : CYP1A1
Synonyms : CYP1A1; cytochrome P450 family 1 subfamily A member 1; DAH1; CYPIA1; cytochrome P450 1A1; CYP1A1 type 1; CYP1A1 type 2; Cytochrome P450 1A1; cytochrome P450, family 1, subfamily A, polypeptide 1
Gene ID : 100686778
UniProt ID : P56590

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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