Description : |
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. |
Source : |
E. coli |
Species : |
Firefly |
Tag : |
N-His |
Form : |
Liquid |
Bio-activity : |
Specific activity is 1×10^10 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at 25 centigrade. |
Molecular Mass : |
63 kDa (571aa) confirmed by MALDI-TOF |
Protein Length : |
1-550 |
AA Sequence : |
MEDAKNIKKGPAPFYPLEDGTAGEQ LHKAMKRYALVPGTIAFTDAHIEVN ITYAEYFEMSVRLAEAMKRYGLNTN HRIVVCSENSLQFFMPVLGALFIGV AVAPANDIYNERELLNSMNISQPTV VFVSKKGLQKILNVQKKLPIIQKII IMDSKTDYQGFQSMYTFVTSHLPPG FNEYDFVPESFDRDKTIALIMNSSG STGLPKGVALPHRTACVRFSHARDP IFGNQIIPDTAILSVVPFHHGFGMF TTLGYLICGFRVVLMYRFEEELFLR SLQDYKIQSALLVPTLFSFFAKSTL IDKYDLSNLHEIASGGAPLSKEVGE AVAKRFHLPGIRQGYGLTETTSAIL ITPEGDDKPGAVGKVVPFFEAKVVD LDTGKTLGVNQRGELCVRGPMIMSG YVNNPEATNALIDKDGWLHSGDLAY WDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDD DAGELPAAVVVLEHGKTMTEKEIVD YVASQVTTAKKLRGGVVFVDEVPKG LTGKLDARKIREILIKAKKGGKSKL |
Purity : |
> 90% by SDS-PAGE |
Applications : |
SDS-PAGE, Enzyme Activity |
Storage : |
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : |
1 mg/mL (determined by Bradford assay) |
Storage Buffer : |
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol |
Official Symbol : |
Luciferase |
Synonyms : |
Luciferase; Luciferin 4-monooxygenase, LuC |
UniProt ID : |
A3KBZ5 |
Publication : |
The effect of spin exchange interaction on protein structural stability (2022)
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