Recombinant Full Length Human AGO1 protein, His-tagged

• Cat.No.: AGO1-10970H
• Product Overview: Full Length Human AGO1 Protein, His Tag is expressed from Baculovirus-Insect cells. It contains AA Glu 2-Ala 857 (Accession # Q9UL18-1). This protein carries a polyhistidine tag at the N-terminus. Predicted N-terminus: Met

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 2-857 aa
• Description: AGO1 (Argonaute-1) is a key protein in the RNA interference (RNAi) pathway. As a core component of the RNA-induced silencing complex (RISC), it binds to small non-coding RNAs like microRNAs (miRNAs) and short interfering RNAs (siRNAs). Unlike its close relative AGO2, AGO1 lacks the slicer activity to cleave target messenger RNAs (mRNAs). Instead, its primary function is to guide RISC to target mRNAs, leading to translational repression and subsequent mRNA degradation. This makes AGO1 crucial for gene silencing and the post-transcriptional regulation of gene expression.
• Form: Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.
• Molecular Mass: The protein has a calculated MW of 99.1 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at: The product MUST be stored at -70 centigrade or lower upon receipt; -70 centigrade for 3 months under sterile conditions. Please avoid repeated freeze-thaw cycles.
• Concentration: 0.2 mg/mL
• Shipping: This product is supplied and shipped with dry ice, please inquire the shipping cost.

Gene Information

• Gene Name: AGO1 argonaute RISC component 1 [ Homo sapiens (human) ]
• Official Symbol: AGO1
• Synonyms: AGO1; argonaute RISC component 1; Q99; EIF2C; hAgo1; EIF2C1; GERP95; NEDLBAS; protein argonaute-1; Golgi Endoplasmic Reticulum protein 95 kDa; argonaute 1, RISC catalytic component; argonaute RISC catalytic component 1; argonaute1; eIF-2C 1; eIF2C 1; eukaryotic translation initiation factor 2C, 1; putative RNA-binding protein Q99
• Gene ID: 26523
• mRNA Refseq: NM_012199
• Protein Refseq: NP_036331
• MIM: 606228
• UniProt ID: Q9UL18