Recombinant Human AGO1 protein, His-tagged

• Cat.No.: AGO1-1754H
• Product Overview: Recombinant Human AGO1 protein(NP_036331.1) (Met 1-Ala 857) was expressed in Insect Cells, with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-857 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Gly, 0.5 PMSF, 0.5mM EDTA, pH 8.0.
• Molecular Mass: The recombinant human AGO1 consists of 875 amino acids and predicts a molecular mass of 99.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.