Recombinant Full Length Human ATG4B Protein, C-Flag-tagged

• Cat.No.: ATG4B-1974HFL
• Product Overview: Recombinant Full Length Human ATG4B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 44.1 kDa
• AA Sequence: MDAATLTYDTLRFAEFEDFPETSEP VWILGRKYSIFTEKDEILSDVASRL WFTYRKNFPAIGGTGPTSDT GWGCMLRCGQMIFAQALVCRHLGRD WRWTQRKRQPDSYFSVLNAFIDRKD SYYSIHQIAQMGVGEGKSIG QWYGPNTVAQVLKKLAVFDTWSSLA VHIAMDNTVVMEEIRRLCRTSVPCA GATAFPADSDRHCNGFPAGA EVTNRPSPWRPLVLLIPLRLGLTDI NEAYVETLKHCFMMPQSLGVIGGKP NSAHYFIGYVGEELIYLDPH TTQPAVEPTDGCFIPDESFHCQHPP CRMSIAELDPSIAVGFFCKTEDDFN DWCQQVKKLSLLGGALPMFE LVEQQPSHLACPDVLNLSLDSSDVE RLERFFDSEDEDFEILSL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Protease
• Protein Pathways: Regulation of autophagy

Gene Information

• Gene Name: ATG4B autophagy related 4B cysteine peptidase [ Homo sapiens (human) ]
• Official Symbol: ATG4B
• Synonyms: APG4B; AUTL1; HsAPG4B
• Gene ID: 23192
• mRNA Refseq: NM_013325.5
• Protein Refseq: NP_037457.3
• MIM: 611338
• UniProt ID: Q9Y4P1