Recombinant Human CBS Protein, GST-Tagged

• Cat.No.: CBS-0471H
• Product Overview: Human CBS full-length ORF (AAH11381.1, 1 a.a. - 551 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2016]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 86.35 kDa
• AA Sequence: MPSETPQAEVGPTGCPHRSGPHSAK GSLEKGSPEDKEAKEPLWIRPDAPS RCTWQLGRPASESPHHHTAPAKSPK ILPDILKKIGDTPMVRINKIGKKFG LKCELLAKCEFFNAGGSVKDRISLR MIEDAERDGTLKPGDTIIEPTSGNT GIGLALAAAVRGYRCIIVMPEKMSS EKVDVLRALGAEIVRTPTNARFDSP ESHVGVAWRLKNEIPNSHILDQYRN ASNPLAHYDTTADEILQQCDGKLDM LVASVGTGGTITGIARKLKEKCPGC RIIGVDPEGSILAEPEELNQTEQTT YEVEGIGYDFIPTVLDRTVVDKWFK SNDEEAFTFARMLIAQEGLLCGGSA GSTVAVAVKAAQELQEGQRCVVILP DSVRNYMTKFLSDRWMLQKGFLKEE DLTEKKPWWWHLRVQELGLSAPLTV LPTITCGHTIEILREKGFDQAPVVD EAGVILGMVTLGNMLSSLLAGKVQP SDQVGKVIYKQFKQIRLTDTLGRLS HILEMDHFALVVHEQIQYHSTGKSS QRQMVFGVVTAIDLLNFVAAQERDQ K
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CBS cystathionine-beta-synthase [ Homo sapiens ]
• Official Symbol: CBS
• Synonyms: CBS; cystathionine-beta-synthase; cystathionine beta-synthase; HIP4; beta-thionase; serine sulfhydrase; methylcysteine synthase
• Gene ID: 875
• mRNA Refseq: NM_000071
• Protein Refseq: NP_000062
• MIM: 613381
• UniProt ID: P35520