Recombinant Full Length Human CDK1 Protein, C-Flag-tagged

• Cat.No.: CDK1-620HFL
• Product Overview: Recombinant Full Length Human CDK1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 33.9 kDa
• AA Sequence: MEDYTKIEKIGEGTYGVVYKGRHKT TGQVVAMKKIRLESEEEGVPSTAIR EISLLKELRHPNIVSLQDVL MQDSRLYLIFEFLSMDLKKYLDSIP PGQYMDSSLVKSYLYQILQGIVFCH SRRVLHRDLKPQNLLIDDKG TIKLADFGLARAFGIPIRVYTHEVV TLWYRSPEVLLGSARYSTPVDIWSI GTIFAELATKKPLFHGDSEI DQLFRIFRALGTPNNEVWPEVESLQ DYKNTFPKWKPGSLASHVKNLDENG LDLLSKMLIYDPAKRISGKM
  ALNHPYFNDLDNQIKKMTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Gap junction, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation

Gene Information

• Gene Name: CDK1 cyclin dependent kinase 1 [ Homo sapiens (human) ]
• Official Symbol: CDK1
• Synonyms: CDC2; CDC28A; P34CDC2
• Gene ID: 983
• mRNA Refseq: NM_001786.5
• Protein Refseq: NP_001777.1
• MIM: 116940
• UniProt ID: P06493