Recombinant Full Length Human CROT Protein, C-Flag-tagged

• Cat.No.: CROT-1243HFL
• Product Overview: Recombinant Full Length Human CROT Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 70 kDa
• AA Sequence: MENQLAKSTEERTFQYQDSLPSLPV PSLEESLKKYLESVKPFANQEEYKK TEEIVQKFQSGIGEKLHQKL LERAKGKRNWLEEWWLNVAYLDVRI PSQLNVNFAGPAAHFEHYWPPKEGT QLERGSITLWHNLNYWQLLR KEKVPVHKVGNTPLDMNQFRMLFST CKVPGITRDSIMNYFRTESEGRSPN HIVVLCRGRAFVFDVIHEGC LVTPPELLRQLTYIHKKCHSEPDGP GIAALTSEERTRWAKAREYLIGLDP ENLALLEKIQSSLLVYSMED SSPHVTPEDYSEIIAAILIGDPTVR WGDKSYNLISFSNGVFGCNCDHAPF DAMIMVNISYYVDEKIFQNE GRWKGSEKVRDIPLPEELIFIVDEK VLNDINQAKAQYLREASDLQIAAYA FTSFGKKLTKNKMLHPDTFI QLALQLAYYRLHGHPGCCYETAMTR HFYHGRTETMRSCTVEAVRWCQSMQ DPSVNLRERQQKMLQAFAKH NKMMKDCSAGKGFDRHLLGLLLIAK EEGLPVPELFTDPLFSKSGGGGNFV LSTSLVGYLRVQGVVVPMVH
  NGYGFFYHIRDDRFVVACSAWKSCP ETDAEKLVQLTFCAFHDMIQLMNST HLTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: CROT carnitine O-octanoyltransferase [ Homo sapiens (human) ]
• Official Symbol: CROT
• Synonyms: COT
• Gene ID: 54677
• mRNA Refseq: NM_021151.4
• Protein Refseq: NP_066974.2
• MIM: 606090
• UniProt ID: Q9UKG9