Recombinant Full Length Human GIT1 Protein, C-Flag-tagged

• Cat.No.: GIT1-1144HFL
• Product Overview: Recombinant Full Length Human GIT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables gamma-tubulin binding activity. Involved in positive regulation of microtubule nucleation and regulation of cytokinesis. Located in several cellular components, including focal adhesion; microtubule cytoskeleton; and mitochondrion. Implicated in attention deficit hyperactivity disorder. Biomarker of Huntington's disease.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 84.2 kDa
• AA Sequence: MSRKGPRAEVCADCSAPDPGWASIS RGVLVCDECCSVHRSLGRHISIVKH LRHSAWPPTLLQMVHTLASN GANSIWEHSLLDPAQVQSGRRKANP QDKVHPIKSEFIRAKYQMLAFVHKL PCRDDDGVTAKDLSKQLHSS VRTGNLETCLRLLSLGAQANFFHPE KGTTPLHVAAKAGQTLQAELLVVYG ADPGSPDVNGRTPIDYARQA GHHELAERLVECQYELTDRLAFYLC GRKPDHKNGHYIIPQMADSLDLSEL AKAAKKKLQALSNRLFEELA MDVYDEVDRRENDAVWLATQNHSTL VTERSAVPFLPVNPEYSATRNQGRQ KLARFNAREFATLIIDILSE AKRRQQGKSLSSPTDNLELSLRSQS DLDDQHDYDSVASDEDTDQEPLRST GATRSNRARSMDSSDLSDGA VTLQEYLELKKALATSEAKVQQLMK VNSSLSDELRRLQREIHKLQAENLQ LRQPPGPVPTPPLPSERAEH TPMAPGGSTHRRDRQAFSMYEPGSA LKPFGGPPGDELTTRLQPFHSTELE DDAIYSVHVPAGLYRIRKGV SASAVPFTPSSPLLSCSQEGSRHTS KLSRHGSGADSDYENTQSGDPLLGL EGKRFLELGKEEDFHPELES LDGDLDPGLPSTEDVILKTEQVTKN IQELLRAAQEFKHDSFVPCSEKIHL AVTEMASLFPKRPALEPVRS
  SLRLLNASAYRLQSECRKTVPPEPG APVDFQLLTQQVIQCAYDIAKAAKQ LVTITTREKKQTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Regulation of actin cytoskeleton

Gene Information

• Gene Name: GIT1 GIT ArfGAP 1 [ Homo sapiens (human) ]
• Official Symbol: GIT1
• Synonyms: p95-APP1
• Gene ID: 28964
• mRNA Refseq: NM_014030.4
• Protein Refseq: NP_054749.2
• MIM: 608434
• UniProt ID: Q9Y2X7