Recombinant Full Length Human GOT1 Protein, C-Flag-tagged

• Cat.No.: GOT1-2051HFL
• Product Overview: Recombinant Full Length Human GOT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.1 kDa
• AA Sequence: MAPPSVFAEVPQAQPVLVFKLTADF REDPDPRKVNLGVGAYRTDDCHPWV LPVVKKVEQKIANDNSLNHE YLPILGLAEFRSCASRLALGDDSPA LKEKRVGGVQSLGGTGALRIGADFL ARWYNGTNNKNTPVYVSSPT WENHNAVFSAAGFKDIRSYRYWDAE KRGLDLQGFLNDLENAPEFSIVVLH ACAHNPTGIDPTPEQWKQIA SVMKHRFLFPFFDSAYQGFASGNLE RDAWAIRYFVSEGFEFFCAQSFSKN FGLYNERVGNLTVVGKEPES ILQVLSQMEKIVRITWSNPPAQGAR IVASTLSNPELFEEWTGNVKTMADR ILTMRSELRARLEALKTPGT WNHITDQIGMFSFTGLNPKQVEYLV NEKHIYLLPSGRINVSGLTTKNLDY VATSIHEAVTKIQ TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism

Gene Information

• Gene Name: GOT1 glutamic-oxaloacetic transaminase 1 [ Homo sapiens (human) ]
• Official Symbol: GOT1
• Synonyms: AST; AST1; SGOT; cCAT; GIG18; cAspAT; ASTQTL1
• Gene ID: 2805
• mRNA Refseq: NM_002079.3
• Protein Refseq: NP_002070.1
• MIM: 138180
• UniProt ID: P17174