Human GOT1 Knockdown Cell Lysate

• Cat.No.: GOT1-347HKCL
• Product Overview: WB-validated GOT1 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 46 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg GOT1 KD HeLa cell lysate
• Protein Pathways: Alanine, Arginine and proline metabolism, aspartate and glutamate metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, Phenylalanine metabolism, tyrosine and tryptophan biosynthesis, Tyrosine metabolism
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: GOT1 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) [ Homo sapiens (human) ]
• Official Symbol: GOT1
• Synonyms: GOT1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); aspartate aminotransferase, cytoplasmic; transaminase A; growth-inhibiting protein 18; glutamate oxaloacetate transaminase 1; GIG18; ASTQTL1
• Gene ID: 2805
• mRNA Refseq: NM_002079
• Protein Refseq: NP_002070
• MIM: 138180
• UniProt ID: P17174