Recombinant Full Length Human HDAC1 Protein, C-Flag-tagged

• Cat.No.: HDAC1-1190HFL
• Product Overview: Recombinant Full Length Human HDAC1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.9 kDa
• AA Sequence: MAQTQGTRRKVCYYYDGDVGNYYYG QGHPMKPHRIRMTHNLLLNYGLYRK MEIYRPHKANAEEMTKYHSD DYIKFLRSIRPDNMSEYSKQMQRFN VGEDCPVFDGLFEFCQLSTGGSVAS AVKLNKQQTDIAVNWAGGLH HAKKSEASGFCYVNDIVLAILELLK YHQRVLYIDIDIHHGDGVEEAFYTT DRVMTVSFHKYGEYFPGTGD LRDIGAGKGKYYAVNYPLRDGIDDE SYEAIFKPVMSKVMEMFQPSAVVLQ CGSDSLSGDRLGCFNLTIKG HAKCVEFVKSFNLPMLMLGGGGYTI RNVARCWTYETAVALDTEIPNELPY NDYFEYFGPDFKLHISPSNM TNQNTNEYLEKIKQRLFENLRMLPH APGVQMQAIPEDAIPEESGDEDEDD PDKRISICSSDKRIACEEEF
  SDSEEEGEGGRKNSSNFKKAKRVKT EDEKEKDPEEKKEVTEEEKTKEEKP EAKGVKEEVKLATRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Adult stem cells, Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer

Gene Information

• Gene Name: HDAC1 histone deacetylase 1 [ Homo sapiens (human) ]
• Official Symbol: HDAC1
• Synonyms: HD1; RPD3; KDAC1; GON-10; RPD3L1
• Gene ID: 3065
• mRNA Refseq: NM_004964.3
• Protein Refseq: NP_004955.2
• MIM: 601241
• UniProt ID: Q13547