Recombinant Full Length Human HFE Protein, C-Flag-tagged

• Cat.No.: HFE-455HFL
• Product Overview: Recombinant Full Length Human HFE Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.7 kDa
• AA Sequence: MGPRARPALLLLMLLQTAVLQGRLL RSHSLHYLFMGASEQDLGLSLFEAL GYVDDQLFVFYDHESRRVEP RTPWVSSRISSQMWLQLSQSLKGWD HMFTVDFWTIMENHNHSKESHTLQV ILGCEMQEDNSTEGYWKYGY DGQDHLEFCPDTLDWRAAEPRAWPT KLEWERHKIRARQNRAYLERDCPAQ LQQLLELGRGVLDQQVPPLV KVTHHVTSSVTTLRCRALNYYPQNI TMKWLKDKQPMDAKEFEPKDVLPNG DGTYQGWITLAVPPGEEQRY TCQVEHPGLDQPLIVIWEPSPSGTL VIGVISGIAVFVVILFIGILFIILR KRQGSRGAMGHYVLAERETRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: HFE homeostatic iron regulator [ Homo sapiens (human) ]
• Official Symbol: HFE
• Synonyms: HH; HFE1; HLA-H; MVCD7; TFQTL2
• Gene ID: 3077
• mRNA Refseq: NM_000410.4
• Protein Refseq: NP_000401.1
• MIM: 613609
• UniProt ID: Q30201