Recombinant Full Length Human IRAK4 Protein, C-Flag-tagged

• Cat.No.: IRAK4-940HFL
• Product Overview: Recombinant Full Length Human IRAK4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51.3 kDa
• AA Sequence: MNKPITPSTYVRCLNVGLIRKLSDF IDPQEGWKKLAVAIKKPSGDDRYNQ FHIRRFEALLQTGKSPTSEL LFDWGTTNCTVGDLVDLLIQNEFFA PASLLLPDAVPKTANTLPSKEAITV QQKQMPFCDKDRTLMTPVQN LEQSYMPPDSSSPENKSLEVSDTRF HSFSFYELKNVTNNFDERPISVGGN KMGEGGFGVVYKGYVNNTTV AVKKLAAMVDITTEELKQQFDQEIK VMAKCQHENLVELLGFSSDGDDLCL VYVYMPNGSLLDRLSCLDGT PPLSWHMRCKIAQGAANGINFLHEN HHIHRDIKSANILLDEAFTAKISDF GLARASEKFAQTVMTSRIVG TTAYMAPEALRGEITPKSDIYSFGV VLLEIITGLPAVDEHREPQLLLDIK EEIEDEEKTIEDYIDKKMND
  ADSTSVEAMYSVASQCLHEKKNKRP DIKKVQQLLQEMTASTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway

Gene Information

• Gene Name: IRAK4 interleukin 1 receptor associated kinase 4 [ Homo sapiens (human) ]
• Official Symbol: IRAK4
• Synonyms: IPD1; IMD67; REN64; IRAK-4; NY-REN-64
• Gene ID: 51135
• mRNA Refseq: NM_016123.4
• Protein Refseq: NP_057207.2
• MIM: 606883
• UniProt ID: Q9NWZ3