Recombinant Full Length Human IRAK4 Protein, C-Flag-tagged

• Cat.No.: IRAK4-940HFL
• Product Overview: Recombinant Full Length Human IRAK4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51.3 kDa
• AA Sequence: MNKPITPSTYVRCLNVGLIRKLSDF IDPQEGWKKLAVAIKKPSGDDRYNQ FHIRRFEALLQTGKSPTSEL LFDWGTTNCTVGDLVDLLIQNEFFA PASLLLPDAVPKTANTLPSKEAITV QQKQMPFCDKDRTLMTPVQN LEQSYMPPDSSSPENKSLEVSDTRF HSFSFYELKNVTNNFDERPISVGGN KMGEGGFGVVYKGYVNNTTV AVKKLAAMVDITTEELKQQFDQEIK VMAKCQHENLVELLGFSSDGDDLCL VYVYMPNGSLLDRLSCLDGT PPLSWHMRCKIAQGAANGINFLHEN HHIHRDIKSANILLDEAFTAKISDF GLARASEKFAQTVMTSRIVG TTAYMAPEALRGEITPKSDIYSFGV VLLEIITGLPAVDEHREPQLLLDIK EEIEDEEKTIEDYIDKKMND
  ADSTSVEAMYSVASQCLHEKKNKRP DIKKVQQLLQEMTASTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway

Gene Information

• Gene Name: IRAK4 interleukin 1 receptor associated kinase 4 [ Homo sapiens (human) ]
• Official Symbol: IRAK4
• Synonyms: IPD1; IMD67; REN64; IRAK-4; NY-REN-64
• Gene ID: 51135
• mRNA Refseq: NM_016123.4
• Protein Refseq: NP_057207.2
• MIM: 606883
• UniProt ID: Q9NWZ3

Reviews

11/16/2021

Its potency and specificity make it a valuable tool for studying specific biological pathways or mechanisms.

03/01/2020

Their technical expertise and experience in the field allow them to provide invaluable guidance and assistance.

07/16/2018

They can offer insights on experimental design, optimal usage protocols, and troubleshoot any challenges that may arise during the course of the trial.

Q&As

What conditions are associated with IRAK4 dysregulation?

Rheumatoid arthritis, lupus, inflammatory bowel diseases, and certain cancers may involve IRAK4.

Are there any ongoing clinical trials involving IRAK4 inhibitors?

Yes, several trials are investigating IRAK4 inhibitors' effectiveness and safety in various diseases.

What are the methods used to target IRAK4 in treatment?

Small molecule inhibitors and monoclonal antibodies are primary methods for modulating IRAK4 activity.

How does the expression of IRAK4 differ in healthy individuals and those with autoimmune diseases?

Individuals with autoimmune diseases often show increased IRAK4 expression compared to healthy individuals.

How does IRAK4 inhibition impact the immune response?

By blocking IRAK4, the overactive immune response in certain diseases can be suppressed.