Recombinant Full Length Human MCL1 Protein, C-Flag-tagged

• Cat.No.: MCL1-337HFL
• Product Overview: Recombinant Full Length Human MCL1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.2 kDa
• AA Sequence: MFGLKRNAVIGLNLYCGGAGLGAGS GGATRPGGRLLATEKEASARREIGG GEAGAVIGGSAGASPPSTLT PDSRRVARPPPIGAEVPDVTATPAR LLFFAPTRRAAPLEEMEAPAADAIM SPEEELDGYEPEPLGKRPAV LPLLELVGESGNNTSTDGSLPSTPP PAEEEEDELYRQSLEIISRYLREQA TGAKDTKPMGRSGATSRKAL ETLRRVGDGVQRNHETAFQGMLRKL DIKNEDDVKSLSRVMIHVFSDGVTN WGRIVTLISFGAFVAKHLKT INQESCIEPLAESITDVLVRTKRDW LVKQRGWDGFVEFFHVEDLEGGIRN VLLAFAGVAGVGAGLAYLIRTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: MCL1 MCL1 apoptosis regulator, BCL2 family member [ Homo sapiens (human) ]
• Official Symbol: MCL1
• Synonyms: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Gene ID: 4170
• mRNA Refseq: NM_021960.5
• Protein Refseq: NP_068779.1
• MIM: 159552
• UniProt ID: Q07820

Reviews

06/20/2022

Another advantage of using MCL1 protein in trials is its potential as a therapeutic agent.

01/16/2022

The manufacturer's support and expertise further enhance the utility of MCL1 in my experimental work, ensuring reliable and high-quality results.

03/23/2016

This collaborative partnership greatly contributes to the success of my research projects and facilitates meaningful contributions to the scientific community.

Q&As

Can targeting MCL1 have implications beyond cancer therapy?

Yes, MCL1 modulation may have therapeutic implications in other diseases, such as neurodegenerative disorders, where apoptosis is a contributing factor.

Are there any diagnostic tools or biomarkers that can assess MCL1 expression in cancer patients?

Immunohistochemistry and molecular assays can be used to measure MCL1 expression levels, providing insights into the prognosis and potential treatment strategies.

How does the understanding of MCL1 function contribute to personalized medicine in cancer treatment?

Understanding MCL1 expression allows for the identification of patients who may benefit from targeted therapies, contributing to personalized treatment strategies.

Are there any ongoing clinical trials exploring MCL1 inhibitors as a therapeutic approach?

Yes, there are several clinical trials investigating the use of MCL1 inhibitors in different cancer types to assess their safety and efficacy.

How does the MCL1 protein interact with other proteins in the Bcl-2 family?

MCL1 interacts with pro-apoptotic and anti-apoptotic proteins in the Bcl-2 family, influencing the balance between cell survival and death.