Recombinant Full Length Human MMP9 Protein, C-Flag-tagged

• Cat.No.: MMP9-1403HFL
• Product Overview: Recombinant Full Length Human MMP9 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 78.3 kDa
• AA Sequence: MSLWQPLVLVLLVLGCCFAAPRQRQ STLVLFPGDLRTNLTDRQLAEEYLY RYGYTRVAEMRGESKSLGPA LLLLQKQLSLPETGELDSATLKAMR TPRCGVPDLGRFQTFEGDLKWHHHN ITYWIQNYSEDLPRAVIDDA FARAFALWSAVTPLTFTRVYSRDAD IVIQFGVAEHGDGYPFDGKDGLLAH AFPPGPGIQGDAHFDDDELW SLGKGVVVPTRFGNADGAACHFPFI FEGRSYSACTTDGRSDGLPWCSTTA NYDTDDRFGFCPSERLYTRD GNADGKPCQFPFIFQGQSYSACTTD GRSDGYRWCATTANYDRDKLFGFCP TRADSTVMGGNSAGELCVFP FTFLGKEYSTCTSEGRGDGRLWCAT TSNFDSDKKWGFCPDQGYSLFLVAA HEFGHALGLDHSSVPEALMY PMYRFTEGPPLHKDDVNGIRHLYGP RPEPEPRPPTTTTPQPTAPPTVCPT GPPTVHPSERPTAGPTGPPS AGPTGPPTAGPSTATTVPLSPVDDA CNVNIFDAIAEIGNQLYLFKDGKYW RFSEGRGSRPQGPFLIADKW PALPRKLDSVFEEPLSKKLFFFSGR QVWVYTGASVLGPRRLDKLGLGADV AQVTGALRSGRGKMLLFSGR RLWRFDVKAQMVDPRSASEVDRMFP GVPLDTHDVFQYREKAYFCQDRFYR RVSSRSELNQVDQVGYVTYD
  ILQCPEDTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Bladder cancer, Leukocyte transendothelial migration, Pathways in cancer

Gene Information

• Gene Name: MMP9 matrix metallopeptidase 9 [ Homo sapiens (human) ]
• Official Symbol: MMP9
• Synonyms: GELB; CLG4B; MMP-9; MANDP2
• Gene ID: 4318
• mRNA Refseq: NM_004994.3
• Protein Refseq: NP_004985.2
• MIM: 120361
• UniProt ID: P14780